Firefly Luc mRNA (m1 ψ substitution)
Croyez Firefly Luciferase mRNA (Cap 1, N1-mψ) expresses luciferase protein derived from the firefly Photinus pyralis. This enzyme facilitates ATP-dependent D-luciferin oxidation, resulting in the production of oxyluciferin—a light-emitting compound upon returning to its ground state. Widely used as a bioluminescent reporter, our mRNA, supplied at 1 mg/ ml, features a Cap 1 structure, poly A tail, and modified nucleotides. The Cap 1 structure ensures efficient translation, while the N1-mψ and poly A tail minimize host cell immune response and enhance mRNA stability. Ideal for gene expression assays, cell viability studies, and in vivo imaging, Croyez Firefly Luciferase mRNA is designed for effective and versatile applications in gene regulation research.
✔️ High sensitivity and low background
✔️ Ideal monitoring tool for in vivo experiments
✔️ High sensitivity and low background
✔️ Ideal monitoring tool for in vivo experiments
mRNA length:
2257 nt
Base composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap modification:
Cap 1 structure
Poly A tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
2257 nt
Base composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap modification:
Cap 1 structure
Poly A tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
Figure 1: Firefly Luc mRNA (m1ψ substitution) was analyzed on a 1% TAE agarose gel at 100 V for 30 minutes.
Marker: Croyez High Range RNA ladder (cat no: CR0005)
